- Product category
Drosophila melanogaster, fruit fly
3D cell culture
- Product format
- Storage conditions
Vapor phase of liquid nitrogen
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Certificate of Analysis Download
To download a certificate of analysis for Schneider's Drosophila Line 2 [D. Mel. (2), SL2] (CRL-1963), enter the lot number exactly as it appears on your product label or packing slip.
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Certificate of Origin Download
To download a certificate of origin for Schneider's Drosophila Line 2 [D. Mel. (2), SL2] (CRL-1963), enter the lot number exactly as it appears on your product label or packing slip.
Certificate of Origin Request
The certificate of origin for that lot of Schneider's Drosophila Line 2 [D. Mel. (2), SL2] (CRL-1963) is not currently available online. Complete this form to request this certificate of origin.
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You can find your account number on your sales order confirmation or order invoice.
This product sheet is not available online. We only provide this product sheet to customers who have purchased this biosafety level 3 product. If you purchased this product, please contact Technical Service for this product sheet.
Safety Data Sheet Download
Open the Safety Data Sheet for this product to download.
If a requested product is not a hazardous chemical, or does not contain any hazardous chemicals, a SDS is not required and therefore will not be provided.
Please check the Product Sheet and Safety Data Sheet Landing pagefor more information.
Check Safety Data Sheet Information
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
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- Specific applications
- This cell line is a suitable transfection host.
- Growth properties
- Mixed: suspension with some loosely adherent cells
The cells have been shown to support the growth of the insect stages of malaria parasites.RefWarburg A, Miller LH. Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521
- Unpacking and storage instructions
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- Schneider's Drosophila Medium, 90%; heat-inactivated fetal bovine serum, 10%. NOTE: the fetal bovine serum should be selected for ability to support the growth of insect cell lines.
This medium is formulated for use without CO2.
- Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
- Thaw the vial by gentle agitation in a 24°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
- Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
- Transfer the vial contents to a 75 cm2 tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio).
- Incubate the culture at 24°C in a suitable incubator.
If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.
- Subculturing procedure
Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Dilute the cell suspension to the desired concentration with fresh medium.Maintain cultures at a cell concentration between 5 x 104 and 4 x 105 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended
Medium Renewal: At the time of subcultivation
Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16°C and 28°C.
Quality control specifications
- Mycoplasma contamination
- Not detected
- Population doubling time
- Approximately 67 hrs
- Deposited as
- Drosophila melanogaster
- IR Schneider
- Patent number
- Schneider's Drosophila Line 2 [D. Mel. (2), SL2] is a cell line exhibiting epithelial-like morphology that was isolated in 1969 from the embryo of a fruit fly. This cell line was deposited IR Schneider.
- Year of origin
- Cross references
GenBank AF250759 Drosophila melanogaster hexokinase gene, partial cds.
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS
Frequently Asked Questions
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Warburg A, Miller LH. Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521
Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067
This line was established in 1969 by I. Schneider from several hundred 20 to 24 hour embryos.
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