Schneider's Drosophila Line 2 [D. Mel. (2), SL2] - CRL-1963 (2023)

The cells have been shown to support the growth of the insect stages of malaria parasites.RefWarburg A, Miller LH. Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

23°C (22-24°C)

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.

1. Thaw the vial by gentle agitation in a 24°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
3. Transfer the vial contents to a 75 cm² tissue culture flask and dilute with the recommended complete culture medium (see the specific batch information for the recommended dilution ratio).
4. Incubate the culture at 24°C in a suitable incubator.

If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information.

Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Dilute the cell suspension to the desired concentration with fresh medium.Maintain cultures at a cell concentration between 5 x 10⁴ and 4 x 10⁵ cells/cm².

Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended

Medium Renewal: At the time of subcultivation

Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16°C and 28°C.

GenBank AF250759 Drosophila melanogaster hexokinase gene, partial cds.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

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